1. ACID-BASE CHEMISTRY
A. Aqueous Solutions
B. Equilibrium Constants
C. Acids and Bases
D. Laboratory Buffers
E. Amino Acids and Peptides
F. Blood Buffers
Practice Problems

2. CHEMISTRY OF BIOLOGICAL MOLECULES
A. Amino Acids; Peptides, and Proteins
B. Carbohydrates
C. Lipids
D. Nucleotides and Nucleic Acids Practice Problems
Practice Problems

3. BIOCHEMICAL ENERGETICS
A. Energy-Yielding and Energy-Requiring Reactions
B. Calculations of Equilibrium Concentrations
C. Oxidation-Reduction Reactions
D. Metabolism and ATP Yield
E. Photosynthetic Phosphorylation
F. Active Transport
G. Enthalpy and Entropy
H. Activation Energy
Practice Problems

4. ENZYMES
A. Enzymes as Biological Catalysts
B. Enzyme Kinetics
C Reaction Order
D Method of Plotting Enzyme Kinetics Data
F Enzyme Inhibition
E Effect of pH on Enzyme Stability and Activity
G Effect of Temperature on l Enzyme Stability and Activity
H. Enzyme Assavs
I Mult substrate Enzymes and Kinetic Mechanisms
J Multisite and Allosteric Enzymes
K Enzyme Turnover
Practice Problems

5 SPECTROPHOTOMETRY AND OTHER OPTICAL METHODS
A Spectrophotometry
B. Fluorometry
C Optical Rotation-Polarimetry
Practice Problems

6 ISOTOPES IN BIOCHEMISTRY
A. Isotopes and Radioactive Decay
B. Solutions of Radioactive Compounds
C. Assays Using Radioactive Substrates
D. Double-Label Analysis
E. Biological Half-Life-Turnover
F. Radioactive Tracer and Dilution Analyses
G. Counting Errors
H. Stable Isotopes
Practice Problems

APPENDICES
I Properties of Commercial Concentrated Solutions of Aids and Bases
II Fractionation with Solid Ammonium Sulfate
III Fractionation with Saturated Ammonium Sulfate Solution
IV. pKa, Values of Acids and Bases Useful in Preparing Buffers
V. Activity Coefficients of Some Ions in Aqueous Solution
VI pKa, Correction Factors for Ionic Strength
VII ionization Constants, pKa pKb, and pI Values of Some Common Amino Acids.
VIII. AG Values for the Hydrolysis of Some Compounds of Biological Importance
IX. Standard Reduction Potentials of Some Oxidation Reduction Half-Reactions
X. Absorption Maxima and Absorption Coefficients of Some Compounds of Biochemical Importance. XI. Specific Rotation of Some Carbohydrates and Derivatives
XII. Radioisotopes Used in Biological Research

ANSWERS TO PRACTICE PROBLEMS

LOGARITHMS

ATOMIC NUMBERS AND ATOMIC WEIGHTS OF THE ELEMENTS

INDEX